Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo

Silva, Ubiana C.; Cuadros-Orellana, Sara; Silva, Daliane R. C.; Freitas-Júnior, Luiz F.; Fernandes, Ana C.; Leite, Laura R.; Oliveira, Christiane A.; Dos Santos, Vera L.

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dc.contributor.author	Silva, Ubiana C.
dc.contributor.author	Cuadros-Orellana, Sara
dc.contributor.author	Silva, Daliane R. C.
dc.contributor.author	Freitas-Júnior, Luiz F.
dc.contributor.author	Fernandes, Ana C.
dc.contributor.author	Leite, Laura R.
dc.contributor.author	Oliveira, Christiane A.
dc.contributor.author	Dos Santos, Vera L.
dc.date.accessioned	2021-11-22T17:37:06Z
dc.date.available	2021-11-22T17:37:06Z
dc.date.issued	2021
dc.identifier.uri	http://repositorio.ucm.cl/handle/ucm/3503
dc.description.abstract	Rock phosphate (RP) is a natural source of phosphorus for agriculture, with the advantage of lower cost and less impact on the environment when compared to synthetic fertilizers. However, the release of phosphorus (P) from RP occurs slowly, which may limit its short-term availability to crops. Hence, the use of P-solubilizing microorganisms to improve the availability of P from this P source is an interesting approach, as microorganisms often perform other functions that assist plant growth, besides solubilizing P. Here, we describe the characterization of 101 bacterial isolates obtained from the rhizosphere and endosphere of maize plants for their P solubilizing activity in vitro, their growth-promoting activity on millet plants cultivated in soil amended with RP, and their gene content especially associated with phosphate solubilization. For the in vitro solubilization assays, two mineral P sources were used: rock phosphate from Araxá (Brazil) mine (AP) and iron phosphate (Fe-P). The amounts of P released from Fe–P in the solubilization assays were lower than those released from AP, and the endophytic bacteria outperformed the rhizospheric ones in the solubilization of both P sources. Six selected strains were evaluated for their ability to promote the growth of millet in soil fertilized with a commercial rock phosphate (cRP). Two of them, namely Bacillus megaterium UFMG50 and Ochrobactrum pseudogrignonense CNPMS2088, performed better than the others in the cRP assays, improving at least six physiological traits of millet or P content in the soil. Genomic analysis of these bacteria revealed the presence of genes related to P uptake and metabolism, and to organic acid synthesis. Using this approach, we identified six potential candidates as bioinoculants, which are promising for use under field conditions, as they have both the genetic potential and the experimentally demonstrated in vivo ability to improve rock phosphate solubilization and promote plant growth.	es_CL
dc.language.iso	en	es_CL
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 Chile	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/cl/	*
dc.source	Frontiers in Microbiology, 11, 574550	es_CL
dc.subject	Bacteria	es_CL
dc.subject	Phosphate	es_CL
dc.subject	Solubilization	es_CL
dc.subject	Maize	es_CL
dc.subject	Plant growth promotion	es_CL
dc.title	Genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo	es_CL
dc.type	Article	es_CL
dc.ucm.indexacion	Scopus	es_CL
dc.ucm.indexacion	Isi	es_CL
dc.ucm.doi	doi.org/10.3389/fmicb.2020.574550	es_CL