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Characterization of microbial communities from gut microbiota of hypercholesterolemic and control subjects

dc.contributor.authorMorales, Cristian Emanuel
dc.contributor.authorRojas, Gabriel Alfonso
dc.contributor.authorRebolledo, Camilo
dc.contributor.authorRojas-Herrera, Marcelo
dc.contributor.authorArias-Carrasco, Raul
dc.contributor.authorCuadros-Orellana, Sara
dc.contributor.authorMaracaja-Coutinho, Vinicius
dc.contributor.authorSaavedra, Kathleen
dc.contributor.authorSánchez Leal, Pamela
dc.contributor.authorLanas, Fernando
dc.contributor.authorSalazar, Luis A.
dc.contributor.authorSaavedra, Nicolas
dc.date.accessioned2023-01-03T19:08:50Z
dc.date.available2023-01-03T19:08:50Z
dc.date.issued2022
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/4327
dc.description.abstractIntroduction: In recent years, several studies have evidenced the importance of the microbiome to host physiology as metabolism regulator, along with its potential role in triggering various diseases. In this study, we analyzed the gut microbiota in hypercholesterolemic (cases) and normocholesterolemic (controls) individuals to identify characteristic microbial signature for each condition. Methods: Stool samples were obtained from 57 adult volunteers (27 hypercholesterolemic and 30 controls). The taxonomic profiling of microbial communities was performed using high-throughput sequencing of 16S rRNA V3-V4 amplicons, followed by data analysis using Quantitative Insights Into Microbial Ecology 2 (QIIME2) and linear discriminant analysis (LDA) effect size (LEfSe). Results: Significant differences were observed in weight, height, body mass index (BMI) and serum levels of triglycerides, total cholesterol and low-density lipoprotein cholesterol (LDL-C) between the groups (p<0.05). LEfSe showed differentially abundant prokaryotic taxa (a=0.05, LDA score > 2.0) in the group of hypercholesterolemic individuals (Methanosphaera, Rothia, Chromatiales, Clostridiales, Bacillaceae and Coriobacteriaceae) and controls (Faecalibacterium, Victivallis and Selenomonas) at various taxonomic levels. In addition, through the application of Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2 (PICRUSt2), the predominance of pathways related to biosynthesis in hypercholesterolemic patients was established, compared to controls in which degradation pathways were predominant. Finally, in the analysis of co-occurrence networks, it was possible to identify associations between the microorganisms present in both studied groups. Conclusion: Our results point out to unique microbial signatures, which likely play a role on the cholesterol metabolism in the studied population.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceFrontiers in Cellular and Infection Microbiology, 12, 943609es_CL
dc.subjectGut microbiotaes_CL
dc.subjectLDL cholesteroles_CL
dc.subjectLEfSe analysises_CL
dc.subjectMicrobial signaturees_CL
dc.subject16S rRNA sequencinges_CL
dc.titleCharacterization of microbial communities from gut microbiota of hypercholesterolemic and control subjectses_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias Agrarias y Forestaleses_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.urifrontiersin.org/articles/10.3389/fcimb.2022.943609/fulles_CL
dc.ucm.doidoi.org/10.3389/fcimb.2022.943609es_CL


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Atribución-NoComercial-SinDerivadas 3.0 Chile
Excepto si se señala otra cosa, la licencia de la publicación se describe como Atribución-NoComercial-SinDerivadas 3.0 Chile