Two new Darwin wasp species of Eiphosoma Cresson, 1865 (E. nigroandinum Fernandes sp. nov. and E. merceae Fernandes sp. nov.) are described from the northwestern Andes of Colombia. Furthermore, we propose the establishment of a new species-group, E. nigroandinum species-group, to encompass these newly described species. Additionally, we expand the geographical distribution of Eiphosoma vitticolle Cresson, 1865, and complement the findings with a map and digital images.