Ver publicación 

Mostrar el registro sencillo de la publicación

Novel apoplastic antifreeze proteins of Deschampsia antarctica as enhancer of common cell freezing media for cryobanking of genetic resources, a preliminary study

dc.contributor.authorShort, Stefania
dc.contributor.authorZamorano, Mauricio
dc.contributor.authorAranzaez-Ríos, Cristian
dc.contributor.authorLee-Estevez, Manuel
dc.contributor.authorDíaz, Rommy
dc.contributor.authorQuiñones, John
dc.contributor.authorUlloa-Rodríguez, Patricio
dc.contributor.authorFigueroa Villalobos, Elías
dc.contributor.authorBravo, León A.
dc.contributor.authorGraether, Steffen P.
dc.contributor.authorFarías, Jorge G.
dc.date.accessioned2024-04-16T19:18:50Z
dc.date.available2024-04-16T19:18:50Z
dc.date.issued2024
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/5312
dc.description.abstractAntifreeze proteins (AFPs) are natural biomolecules found in cold-adapted organisms that lower the freezing point of water, allowing survival in icy conditions. These proteins have the potential to improve cryopreservation techniques by enhancing the quality of genetic material postthaw. Deschampsia antarctica, a freezing-tolerant plant, possesses AFPs and is a promising candidate for cryopreservation applications. In this study, we investigated the cryoprotective properties of AFPs from D. antarctica extracts on Atlantic salmon spermatozoa. Apoplastic extracts were used to determine ice recrystallization inhibition (IRI), thermal hysteresis (TH) activities and ice crystal morphology. Spermatozoa were cryopreserved using a standard cryoprotectant medium (C+) and three alternative media supplemented with apoplastic extracts. Flow cytometry was employed to measure plasma membrane integrity (PMI) and mitochondrial membrane potential (MMP) postthaw. Results showed that a low concentration of AFPs (0.05 mg/mL) provided significant IRI activity. Apoplastic extracts from D. antarctica demonstrated a cryoprotective effect on salmon spermatozoa, with PMI comparable to the standard medium. Moreover, samples treated with apoplastic extracts exhibited a higher percentage of cells with high MMP. These findings represent the first and preliminary report that suggests that AFPs derived from apoplastic extracts of D. antarctica have the potential to serve as cryoprotectants and could allow the development of novel freezing media.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceBiomolecules, 14(2), 174es_CL
dc.subjectAntifreeze proteinses_CL
dc.subjectApoplastic extractes_CL
dc.subjectIce recrystallization inhibitiones_CL
dc.subjectThermal hysteresises_CL
dc.subjectCryoprotectantes_CL
dc.titleNovel apoplastic antifreeze proteins of Deschampsia antarctica as enhancer of common cell freezing media for cryobanking of genetic resources, a preliminary studyes_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias Agrarias y Forestaleses_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.urimdpi.com/2218-273X/14/2/174es_CL
dc.ucm.doidoi.org/10.3390/biom14020174es_CL


Ficheros en la publicación

FicherosTamañoFormatoVer

No hay ficheros asociados a esta publicación.

Esta publicación aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo de la publicación

Atribución-NoComercial-SinDerivadas 3.0 Chile
Excepto si se señala otra cosa, la licencia de la publicación se describe como Atribución-NoComercial-SinDerivadas 3.0 Chile