Ver publicación 

Mostrar el registro sencillo de la publicación

Characterization of the conversion system of natural rubber to poly(3-Hydroxyalkanoate) in Piscinibacter gummiphilus strain NS21T

dc.contributor.authorTamamura, Masaki
dc.contributor.authorGibu, Namiko
dc.contributor.authorToda, Tomoyuki
dc.contributor.authorTakenaka, Katsuhiko
dc.contributor.authorHang, Dam Thuy
dc.contributor.authorLan Huong, Nguyen
dc.contributor.authorAndler, Rodrigo
dc.contributor.authorKasai, Daisuke
dc.date.accessioned2024-10-03T14:09:44Z
dc.date.available2024-10-03T14:09:44Z
dc.date.issued2024
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/5689
dc.description.abstractPoly(3-hydroxyalkanoate) (PHA), a bacteria-synthesized biodegradable polyester, is a useful alternative to fossil resources, and current systems for its production rely predominantly on edible resources, raising concerns about microbial competition for nutrients. Therefore, we investigated mechanisms underlying PHA production from non-edible resources by Piscinibacter gummiphilus strain NS21T. Strain NS21T can utilize natural rubber as a carbon source on solid media and potentially produces PHA. Gas chromatography and nuclear magnetic resonance analyses of NS21T cell extracts revealed the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(3-hydroxybutyrate) from natural rubber and glucose, respectively. Transcriptional analysis suggested that phaC is involved in PHA production. An increased PHBV accumulation rate under nitrogen-limiting conditions indicates the potential of this strain to be used as a PHBV production enhancement strategy. Furthermore, the disruption of PHA depolymerase genes resulted in enhanced PHA production, indicating the involvement of these genes in PHA degradation. These findings highlight the potential of NS21T for PHBV production from natural rubber, a non-edible resource.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceNew Biotechnology, 84, 1-8es_CL
dc.subjectPiscinibacter gummiphilus NS21Tes_CL
dc.subjectPoly(3-hydroxyalkanoate) productiones_CL
dc.subjectDeproteinized natural rubberes_CL
dc.subjectPHA depolymerase genees_CL
dc.subjectNitrogen-limitation strategyes_CL
dc.titleCharacterization of the conversion system of natural rubber to poly(3-Hydroxyalkanoate) in Piscinibacter gummiphilus strain NS21Tes_CL
dc.typeArticlees_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.urisciencedirect.ucm.elogim.com/science/article/pii/S1871678424005417?via%3Dihubes_CL
dc.ucm.doidoi.org/10.1016/j.nbt.2024.08.507es_CL


Ficheros en la publicación

FicherosTamañoFormatoVer

No hay ficheros asociados a esta publicación.

Esta publicación aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo de la publicación

Atribución-NoComercial-SinDerivadas 3.0 Chile
Excepto si se señala otra cosa, la licencia de la publicación se describe como Atribución-NoComercial-SinDerivadas 3.0 Chile