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Generating transcriptomic resources in the teleost fish black cusk-eel (Genypterus maculatus) to evaluate thermal stress in the liver under a climate change scenario

dc.contributor.authorDettleff, Phillip
dc.contributor.authorFuentes, Marcia
dc.contributor.authorGonzalez, Pamela
dc.contributor.authorAedo, Jorge
dc.contributor.authorZuloaga, Rodrigo
dc.contributor.authorManuel Estrada, Juan
dc.contributor.authorMolina, Alfredo
dc.contributor.authorValdes, Juan Antonio
dc.date.accessioned2025-06-12T14:19:31Z
dc.date.available2025-06-12T14:19:31Z
dc.date.issued2025
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/6131
dc.description.abstractThe black cusk-eel (Genypterus maculatus) is a native fish that is relevant for traditional Chilean fisheries and has aquaculture potential. However, the genomic information and the biological knowledge related to the effect of thermal stress response are limited. This study generated the first de novo transcriptome assembly of the liver of G. maculatus and investigated the hepatic response to thermal stress in the G. maculatus. The de novo assembly resulted in 26,620 annotated transcripts, with an N50 of 2297, and a GC% of 49.76%. BUSCO analysis showed 97.1% and 75.7% complete orthologous (Metazoa and Actinopterygi, respectively). Functional annotation showed a total of 55,556 GO terms, with 26,128 annotations on biological process, 15,225 annotations on molecular functions, and 14,213 annotations on cellular component. The RNA-seq analysis revealed 94 differentially expressed transcripts in response to thermal stress, with 64 downregulated and 30 upregulated transcripts. The enrichment analysis showed biological processes related to double-strand break repair via homologous recombination, reciprocal meiotic recombination, and DNA repair. A significant increase in cortisol levels with no significant difference activity of hepatic enzymes (ALT, AST, AP) due to thermal stress was observed. Also, an increase in DNA damage (AP sites formation) and lipid peroxidation (HNE protein adducts) in the liver due to thermal stress was observed. The differentially expressed transcripts were validated using qPCR, confirming the RNA-seq results. The findings provide valuable genomic information for G. maculatus and highlight the physiological and molecular responses to thermal stress in the species under the context of climate change.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceFish Physiology and Biochemistry, 51(2), 75es_CL
dc.subjectRNA-seqes_CL
dc.subjectHigh temperaturees_CL
dc.subjectDNA damagees_CL
dc.subjectHepatic responsees_CL
dc.subjectOxidative stresses_CL
dc.subjectDe novo assemblyes_CL
dc.titleGenerating transcriptomic resources in the teleost fish black cusk-eel (Genypterus maculatus) to evaluate thermal stress in the liver under a climate change scenarioes_CL
dc.typeArticlees_CL
dc.ucm.facultadFacultad de Ciencias Básicases_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.urispringerlink.ucm.elogim.com/article/10.1007/s10695-025-01486-yes_CL
dc.ucm.doidoi.org/10.1007/s10695-025-01486-yes_CL


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Atribución-NoComercial-SinDerivadas 3.0 Chile
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