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Development and application of novel SSR markers to assess the genetic diversity and population structure of phacelia secunda along an altitudinal gradient in the central Chile Andes

dc.contributor.authorTorres-Díaz, Cristian
dc.contributor.authorOrtíz-Sepúlveda, Ana
dc.contributor.authorValladares, Moisés A.
dc.contributor.authorFarias-Cantillana, Darío
dc.contributor.authorMolina-Montenegro, Marco A.
dc.contributor.authorBallesteros, Gabriel I.
dc.date.accessioned2025-06-12T14:24:11Z
dc.date.available2025-06-12T14:24:11Z
dc.date.issued2025
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/6140
dc.description.abstractPhacelia secunda J.F. Gmel. (Boraginaceae) is a widely distributed insect-pollinated perennial herb. In central Chile (33° S), it occurs from the sea level up to 3600 m in the Andes, exhibiting broad morphological variation. In this study, we developed and characterized novel polymorphic microsatellites for this species, using an Illimina MiSeq sequencing platform. Nineteen polymorphic loci were obtained, with alleles numbers ranging from 3 to 13 per locus (mean = 5.84). Observed (HO) and expected heterozygosities (HE) ranged from 0.050 to 0.900 and from 0.049 to 0.825, respectively. These markers were applied to assess the genetic diversity and population structure along an altitudinal spanning from 1600 to 3600 m. The highest elevation population exhibited significantly lower within-population genetic diversity compared to lower-elevation populations. Significant population differentiation was observed along the gradient. Gene flow estimates support a stepping-stone like mode of migration, with greater exchange between adjacent elevations. These new microsatellites provide a valuable tool for elucidating the influence of altitude on genetic diversity and structure, and for evaluating the roles of local adaptation and phenotypic plasticity in shaping population variation.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourcePlants, 14(7), 1135es_CL
dc.subjectHigh-elevation ecosystemses_CL
dc.subjectHigh-throughput sequencinges_CL
dc.subjectMountain ecosystemses_CL
dc.subjectAlpinees_CL
dc.subjectAndeanes_CL
dc.subjectSSRses_CL
dc.titleDevelopment and application of novel SSR markers to assess the genetic diversity and population structure of phacelia secunda along an altitudinal gradient in the central Chile Andeses_CL
dc.typeArticlees_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.urimdpi.com/2223-7747/14/7/1135es_CL
dc.ucm.doidoi.org/10.3390/plants14071135es_CL


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